Regulatory

Part:BBa_K3745035

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-30)


LacI promoter and LacI

This part encodes LacI promoter and LacI, which could express repressor protein to bind with lac operator and repress transcription. However, when lactose or IPTG is present, the repressor will first combine with lactose or IPTG, and could no longer restrict the transcription of genes downstream of the operon.

In our project, it is used to control the expression on rhlA,B,C, and protein scaffold.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None